Western blot was performed using Anti-F1A alpha Polyclonal Antibody (Product # PA5-19955) and a 70 kDa band corresponding to Protein fem-1 homolog B was observed across Mouse Testis, Mouse Heart and Rat Testis, but not in Mouse Ovary and Rat Ovary. Tissue extracts (30 µg lysate) of Mouse Testis (Lane 1), Mouse Heart (Lane 2), Mouse Brain (Lane 3), Mouse Ovary (Lane 4), Rat Testis (Lane 5) and Rat Ovary (Lane 6) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX), 10 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:10,000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).